Biomedical Proteomics
Don’t work in the dark. Get the full picture with proteomics – we provide trusted expertise and next level analyses to fuel more impactful basic research or discover and implement new diagnostic markers for clinical studies
About
From consultation and experimental design to high impact publications, we can help take your research or clinical studies to the next level. You don’t need to know how proteomics works to benefit from what this technology can provide.
Mass Spectrometry (MS) is an extremely powerful analytical technique that supports modern day biomolecular science, making MS a valuable tool in understanding the causes of disease, identifying biomarkers, including diagnostic and clinical outcome markers, with the ability to test blood, tissue, or other biological samples. Every disease has a marker, whether its indicators of dementia or genetic eye disease in blood, we can help you find what you’re looking for.
Our Services
Untargeted proteomics and phosphoproteomics are the two main services performed at the facility, using highly developed workflows that offer a high depth of analysis. We also perform targeted protein analyses, custom protein analyses, and custom metabolite analyses. Our established workflows with the Bioinformatics team at CMRI helps to get the most from your data in the shortest time possible. Please contact us to discuss your project needs, and we can design a solution appropriate for you.
Techniques and Technologies
Mass spectrometry systems:
The facility has an Astral Orbitrap and a Q Exactive Plus quadrupole-orbitrap mass spectrometry systems. The Astral Orbitrap is state-of-the-art, offering very rapid and very sensitive analysis of biomolecules. The Astral is allowing the analysis of difficult and precious patient samples that were previously too challenging. The Q Exactive Plus is a valuable work horse system that supports high throughput analysis for less cutting-edge applications.
Multiple equipment items to support mass spectrometry:
A Vanquish Neo UHPLC system supports multiple off-line chromatography workflows with ultraviolet peptide detection and fraction collection to allow greater depth of proteome analysis.
A Laser Puller Sutter Instrument P-2000 and Nanobaume capillary column packer allow the production of nano-flow chromatography columns with a pulled tip. This allows zero dead volume between the chromatography material and the electrospray emitter, which enables very high-resolution chromatography and sensitive MS detection.
Independent Use or Full Service
Full service: We offer full-service analysis of samples from experimental design to data processing and interpretation of data. It is crucial to design the right mass spectrometry experiments to meet your needs. We have the expertise to guide you. We also carry out sample prep and analysis, and can work seamlessly with you and our Bioinformatics team on computational analyses and modelling to ensure you get quality outcomes. We aim to make every project result in a publication.
Training and Self- Service: The facility offers training so that researchers can become independent users of the MS equipment and use the adjoining wet laboratory space. We can train you or any member of your team, so they can carry out the experiments in our facility using a simple cost per use fee structure
Trusted Expertise
Dr Mark Graham, Lead Scientist for Biomedical Proteomics, has over 20 years experience in proteomics and specialises in phosphoproteomics, the best tool for mechanistic insights. Let him help you design the right experiment to get publishable results.
Just contact us for a consultation.
“As a researcher, I knew what I wanted, but mass spectrometry was an unknown. I got the full service and left all the MS setup to Mark.
Biomedical Proteomics Customer
Trusted Expertise
Dr Mark Graham
Biomedical Proteomics Facility Lead
Dr Mark Graham obtained his Bachelor of Science and PhD degree at The University of Newcastle. During his PhD, he was an early user of proteomics technology for the analysis of proteins/enzymes. Proteomics allows proteins to be well-defined at the molecular level. This approach enabled the discovery of how a particular enzyme interpreted phosphorylation-based signalling mechanisms from different environmental queues, to alter enzyme activity. Since joining CMRI in 2002, Dr Graham has studied neurotransmission using proteomics technology, but has also collaborated in cancer research. His research focused on synaptic vesicle endocytosis, a process that is used in neurons to replenish supply of neurotransmitter filled vesicles. He also studied phosphorylation-based signalling that regulates neurotransmitter release as mechanisms that are involved in homeostatic plasticity.
Selected Recent Biomedical Proteomics Facility Publications
Müller, J. A., J. Betzin, J. Santos-Tejedor, A. Mayer, A. M. Oprişoreanu, K. Engholm-Keller, I. Paulußen, P. Gulakova, T. D. McGovern, L. J. Gschossman, E. Schönhense, J. R. Wark, A. Lamprecht, A. J. Becker, A. J. Waardenberg, M. E. Graham, D. Dietrich, and S. Schoch. “A Presynaptic Phosphosignaling Hub for Lasting Homeostatic Plasticity.” Cell Rep 39, no. 3 (Apr 19 2022): 110696. https://dx.doi.org/10.1016/j.c….
Fernando, M., S. Lee, J. R. Wark, D. Xiao, B. Y. Lim, M. O’Hara-Wright, H. J. Kim, G. C. Smith, T. Wong, E. T. Teber, R. R. Ali, P. Yang, M. E. Graham, and A. Gonzalez-Cordero. “Differentiation of Brain and Retinal Organoids from Confluent Cultures of Pluripotent Stem Cells Connected by Nerve-Like Axonal Projections of Optic Origin.” Stem Cell Reports (Apr 19 2022). https://dx.doi.org/10.1016/j.s….
Almazi, J. G., M. Alomari, L. Belov, O. G. Best, Y. Shen, M. E. Graham, S. P. Mulligan, and R. I. Christopherson. “Fludarabine Nucleoside Induces Major Changes in the P53 Interactome in Human B-Lymphoid Cancer Cell Lines.” Nucleosides Nucleotides Nucleic Acids (Dec 10 2021): 1-7. https://dx.doi.org/10.1080/152….
Fan, X., V. P. Masamsetti, J. Q. Sun, K. Engholm-Keller, P. Osteil, J. Studdert, M. E. Graham, N. Fossat, and P. P. Tam. “Twist1 and Chromatin Regulatory Proteins Interact to Guide Neural Crest Cell Differentiation.” Elife 10 (Feb 8 2021). https://dx.doi.org/10.7554/eLi….
Fan, X., A. J. Waardenberg, M. Demuth, P. Osteil, J. Sun, D. A. F. Loebel, M. Graham, P. P. L. Tam, and N. Fossat. “Twist1 Homodimers and Heterodimers Orchestrate Lineage-Specific Differentiation.” Mol Cell Biol (Mar 16 2020). https://dx.doi.org/10.1128/mcb….
Engholm-Keller, K., A. J. Waardenberg, J. A. Muller, J. R. Wark, R. N. Fernando, J. W. Arthur, P. J. Robinson, D. Dietrich, S. Schoch, and M. E. Graham. “The Temporal Profile of Activity-Dependent Presynaptic Phospho-Signalling Reveals Long-Lasting Patterns of Poststimulus Regulation.” PLoS Biol 17, no. 3 (Mar 2019): e3000170. https://dx.doi.org/10.1371/jou….
Engholm-Keller, Kasper, Nicolai Bache, Sushma R. Rao, Jesse R. Wark, Martin R. Larsen, Phillip J. Robinson, and Mark E. Graham. “Affinity Proteomics for Interactome and Phosphoproteome Screening in Synaptosomes.” In Synaptosomes. Edited by Kathryn M. Murphy. New York, NY: Springer New York, 2018.
Graham, M. E., M. F. Lavin, and S. V. Kozlov. “Identification of Atm Protein Kinase Phosphorylation Sites by Mass Spectrometry.” Methods Mol Biol 1599 (2017): 127-44. https://dx.doi.org/10.1007/978….
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