Single Cell Analytics
Discover more than you thought possible. Discover 10X more.
About
Single cell RNA sequencing is one of the most popular genomic tools used to discover and analyse rare cell types, understand transcriptome heterogeneity within cells and tissues, and assess the influence of environmental stimuli at an RNA level. The diversity of cell populations giving rise to a unique tissue microenvironment that is deserving of individualised analysis.
The Single Cell Analytics Facility at CMRI uses powerful 10X Genomics single cell analytics tools to give you individual RNA seq data sets from individual cells. Our expertise in single cell analytics tools—coupled with individualised support and end-to-end services like custom built bioinformatics tools found nowhere else—means you will not only get exceptional data outcomes but exceptional answers to questions you haven’t even asked yet.
Research Applications Include:
• Tissue and Cell Profiling • Immunology • Embryology • Cancer Research • Disease Biology • and more!
Why Choose Us?
- • Our custom assay optimisation means personalised support to ensure every sample provides exceptional data outcomes. Investing in single cell analytics means generating data that you can continue to mine for more information and generate multiple publications – you’ll start with one question and come away with 10x more answered
- Our in Multiome kits allows you to simultaneously profile gene expression and open chromatin from the same cell. This means greater power to characterize cell types and states and uncover gene regulatory programs.
- We offer Visium Spatial Gene Expression, a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context to discover novel insights into normal development, disease pathology, and clinical translational research
- Tap into our bioinformatics support to get more from your data than the standard analyses—we can help you discover more than you dreamed possible.
- We are experienced at working with range of sample types and have access to CellBank, whose services and expertise can ensure your cells maintain viability and quality needed for low noise and better single cell RNAseq results.
- We offer fast turnaround. Just contact us for a quote and completion estimate.
Our Services
Common Services
- Single Cell Gene Expression 3’ v3.1 Dual Index Kit
- Single Cell Immune Profiling 5’ v1.1 Single Index and v2 Dual Index Kit, human or mouse T and B Cells
- Single Cell Multiome ATAC and Gene Expression Kit
- Visium Spatial Gene Expression Kit
Experienced Sample Prep
We work with a range of cell types and preparations including:
•Nuclei and whole cell prep
•Fresh and frozen samples
•CRISPR edited cells
10X Chromium 3’ Gene Expression service
This service includes:
• Cell counts and viability processing
• Cell isolation and barcoding using Gel Bead-In Emulsion (GEM) technology
• Post GEM RT Clean up and cDNA amplification
• Gene Expression Library construction
This Chromium Single Cell Gene Expression Solution allows researchers to:
• Identify and characterise rare cell types
• Analyse and evaluate the attribute of cellular heterogeneity in your biological system
• Evaluate mRNA and cell surface protein expression profiles within the same cell
We also offer expansions of 3’ including cell multiplexing, and CRISPR edited cell services.
Looking for something else?
Other 10X solutions are available
Please contact staff at CMRI’s Single Cell Analytics Facility to discuss your needs.
Full service from cell samples to sequencing
The Single Cell Analytic Facility at Children’s Medical Research Institute (CMRI) is highly experienced and will work with you to ensure you receive highly accurate and reproducible results from your samples. What’s more, we can coordinate with CMRI’s CellBank facility to prepare your cell samples for the single-cell 10X Chromium service. Higher quality cells yield higher quality results. We can accept and process cells in frozen vials or growing cultures. You may wish to consider using our other cell bank services in conjunction with the single-cell 10X Chromium service.
Sequencing. We send your samples directly for sequencing to your preferred sequencing company or return samples for your own arrangements.
“The Single Cell Analytics Facility has enabled a streamlined analysis of our stem cell-derived organoids. We are delighted to have this service at hand and the staff couldn’t be more helpful.”
Dr Anai Gonzalez Cordero
What people are saying
“The CMRI’s Single Cell Analytics Facility provides efficient, timely, and cost effective services.”
“Megan is extremely helpful and very flexible when it comes to different experimental designs. She is a delight to work with!”
“Megan has been very helpful in troubleshooting and optimising my experiment and workflow.”
Equipment Available
10X Genomics Chromium Controller
The 10X Genomics Chromium Controller is a high throughput single-cell molecular barcoding system. This instrument allows researchers to go beyond traditional gene expression analysis to characterise cell populations, cell types and cell states on a cell-by-cell basis. The Single Cell Analytics Facility can prepare samples for 3’ Gene Expression assays using the 10X Chromium Controller. This High-throughput automated barcoding and library construction system enables powerful RNA and DNA sequencing applications. The 10X Chromium Controller is driven by 10X Genomics GemCode Technology and enables the isolation of up to 80,000 individual cells in a single run.
Fluidigm C1™ system
The Fluidigm C1™ system provides a quick and easy work flow for the isolation, extraction, reverse transcription, and pre-amplification of up to 800 single cells within a population using integrated fluidic circuits (IFC). Visualisation of capture sites the IFC allows cell viability and capture quality to be assessed. After visualisation, cells are automatically lysed
Biomark™ HD system
The Biomark™ HD system is a high-throughput PCR cycler capable of running up to 96 samples against 96 gene sets in less than 24 hours. It may be used with a wide variety of sample types and chemistry to accommodate most genomic applications. It is also compatible with the Fluidigm C1™ system. The integrated fluidic circuits (IFCs) are available in a range of sizes and allow PCR reactions in nanoliter volumes. The Biomark™ HD system runs IFCs in either real-time or end-point read modes, bringing flexible, efficient and economical PCR solutions to a range of applications including single-cell analysis.
Training for Fluidigm C1™ and Biomark™ HD system
Single Cell Analytics Facility staff can provide full hands-on training for researchers requiring access to these instruments.
Trusted Expertise
Megan Weatherstone
Single Cell Analytics Facility Lead
Megan can help you get the best results. Reach out to her for a consultation.